PUBLISHED LABORATORY MANUALS & TEXTBOOKS

Introduction to Biomanufacturing Textbook
1st Edition Textbook Now Available!
This textbook covers Facilities, Metrology, Validation, EH&S, Operational Excellence, Quality Assurance, Microbiological Control, Quality Control Biochemistry, Upstream Processing, Downstream Processing, Process Development, and includes a Master Glossary.
FULL COLOR VERSION

• 508 Pages (Full Color)
• Retail PRINT Price $149.⁰⁰
• View Table of Contents PDF
• Go To Publisher's PRINT Version Page

Biomanufacturing Laboratory Manual
This comprehensive Global Biomanufacturing Curriculum laboratory manual was created and tested by the Northeast Biomanufacturing Center and Collaborative. This NSF ATE funded project is dedicated to building infrastructure for Biomanufacturing education and training, and the workforce.
FULL COLOR VERSION

• 398 Pages (Full Color)
• Retail PRINT Price $134.³⁰
• View Table of Contents PDF
• Go To Publisher's PRINT Version Page

CHO - tPA Core Production System Lab Manual
A Biomanufacturing laboratory manual focusing on one of three NBC² Core Production Systems utilizing recombinant cells to make proteins-of-interest. This manual focuses on tissue Plasminogen Activator (tPA) secreting CHO cells. Hands-on equipment and processes covered within include Upstream and Downstream Processing and Quality Control Biochemistry SOPs along with a Quality Assurance Batch Record. This laboratory manual was created and tested by the Northeast Biomanufacturing Center and Collaborative. This NSF ATE funded project is dedicated to building infrastructure for Biomanufacturing education and training, and the workforce.

• 98 Pages (Full Color)
• Retail PRINT Price $39.⁹⁵
• Retail DOWNLOAD Price $12.⁰⁰
• View Table of Contents PDF
• Go To Publisher's PRINT Version Page

Pichia pastoris - HSA Core Production System
A Biomanufacturing laboratory manual focusing on one of three NBC² Core Production Systems utilizing recombinant cells to make proteins-of-interest. This manual focuses on Human Serum Albumin (HSA) secreting Pichia pastoris cells. Hands-on equipment and processes covered within include Upstream and Downstream Processing and Quality Control Biochemistry SOPs along with a Quality Assurance Batch Record. This laboratory manual was created and tested by the Northeast Biomanufacturing Center and Collaborative. This NSF ATE funded project is dedicated to building infrastructure for Biomanufacturing education and training, and the workforce.

• 108 Pages (Full Color)
• Retail PRINT Price $39.⁹⁵
• Retail DOWNLOAD Price $14.⁰⁰
• View Table of Contents PDF
• Go To Publisher's PRINT Version Page

Escherichia coli - GFP Core Production System
A Biomanufacturing laboratory manual focusing on one of three NBC² Core Production Systems utilizing recombinant cells to make proteins-of-interest. This manual focuses on Green Fluorescent Protein (GFP) producing Escherichia coli cells. This laboratory manual is meant for use in introductory biotechnology courses at the high school and community college levels. It covers hands-on equipment and process SOPs for the production of GFP within E. coli. Students will learn basic laboratory skills such as micropipetting, media and buffer preparation, and serial dilutions. This laboratory manual was created and tested by the Northeast Biomanufacturing Center and Collaborative. This NSF ATE funded project is dedicated to building infrastructure for Biomanufacturing education and training, and the workforce.

• 38 Pages (Full Color)
• Retail PRINT Price $29.⁹⁵
• Retail DOWNLOAD Price $9.⁰⁰
• View Table of Contents PDF
• Go To Publisher's PRINT Version Page

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HANDS-ON BIOMANUFACTURING TECHNICIAN LABORATORY MODULES:
The following education and training modules provide additional immediately available resources to support biomanufacturing education and training, and the workforce:

POSTERS
Biofuels    15MB .jpg file format
A large format hi resolution file covering 4 aspects of the Biofuels industry. These areas include; 'Growing Oil in Microalgae', 'Bioethanol from Corn', 'Growning Hydrogen in Bacteria' and 'Bioethanol from Cellulose'. Developed from content pioneered by education and industry partners of NBC² including Finger Lakes Community College, MiraCosta College and Bio-Rad.

Manufacturing Training to support the Bio-economy    4MB .pdf file format
A large format hi resolution file developed for the AAAS Community College Forum on Biotechnology Education on February 17, 2011. Assembled by NBC² Co-PI Tom Burkett of the Community College of Baltimore County with input and contributions from various partners in education and industry. Content is focussed primarily on the role of the NBC² as it relates to biomanufacturing training.

Facilities:
Tour of a Pilot Plant for Biopharmaceutical Protein Production
Tour of the Media and Buffer Prep Area
Tour of the Upstream Processing Area
Tour of the Downstream Processing Area
All the above are best viewed at:
http://gallery.me.com/mastercaptain#gallery

Environmental Health and Safety:
Root Cause Analysis Program

Validation:
Bioreactor Vessel Sterilization Validation Protocol
Forward Flow Filter Integrity Test for TFF System Protocol
UV-VIS Spectrophotometer Method Validation Protocol

Upstream Processing:
SOP: Large-Scale Batch Culture of E. coli Recombinant for Jellyfish Green Fluorescent Protein (in process)
Batch Record: Large-Scale Batch Culture of E. coli Recombinant for Jellyfish Green Fluorescent Protein (in process)

Pichia pastoris BioFlo3000 Inoculation Video

Large Scale Bioreactor Video from FAS Biopharma Collaboration
A training video developed by NBC2 while at the FAS - Biopharma Training Facility in County Cork, Ireland 2008
Time = 6:50 File size = 21 meg
View or download the Web video here.
Time = 6:50 File size = 134 meg
View or download Full Screen video here.

Downstream Processing:
SOP: Protein A Chromatography of IgG
SOP: Preparation of Protein A Chromatography Column
SOP: Biologic LP Protein A Chromatography Equipment SOP
SOP: Programming of Biologic LP Chromatography System for
      Protein A Chromatography
SOP: Ion Exchange Chromatography of GFP
SOP: Hydrophobic Interaction Chromatography of GFP
SOP: Gel Filtration Chromatography of GFP

Virtual Chromatography Laboratory based on Actual Chromatography Laboratory

Quality Control – Microbiology:
SOP: BioLog
SOP: BioLog Turbidimeter

Quality Control – Biochemistry:
SOP: HPLC Equipment SOP for Analytical Chromatography of GFP
SOP: HPLC Process SOP for Analytical Chromatography of GFP

Biomanufacturing for High Schools:
Process Logic Control Using Robo-Lego – ROBOLAB
Curriculum Outline – 15 Week Introduction to the Bioeconomy (Biotechnology and Biomanufacturing) with Power Points and Hands-On Labs (in process)

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BIOMAN JOURNALS, NEWSLETTERS & REPORTS:

• BIOMAN 2012
• BIOMAN 7th Annual Biomanufacturing Conference, Alamance CC, Graham, NC (coming soon)
• BIO 2012 Community College Program
• 9th Annual Community College Program Day, Boston, MA
• BIOMAN 2011
• BIOMAN 6th Annual Biomanufacturing Conference, MiraCosta College, Oceanside, CA
• BIOMAN News
• Biomanufacturing News from the Nation, May 2011
• BIOMAN 2010
• BIOMAN 5th Annual Biomanufacturing Conference, Ivy Tech Community College, Bloomington, IN
• BIOMAN Journal 2007
• First Edition of the NBC2 Biomanufacturing Journal

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BIOMAN KEYNOTE VIDEO RESOURCE:

• BIOMAN 2006
1. Miguel Oliazola - Growing Products in Microalgae: Nutriceuticals to Human Proteins
2. Janet Paulson - From Corn to Amino Acids and Ethanol
3. Tim Charlebois - Technology and Opportunities in Mammalian Cell Culture
4. Michael Cicio - Today’s Biologics: From Bench Top to Bottle.
5. Cynthia Sarnoski - Centrality of Quality Control to the Biopharmaceutical Operation


• BIOMAN 2007
1. Stacia Sower - Prehistoric Fish and Genomes: Biological Implications
2. Mark Laser - Cellulosic Ethanol Production
3. Thomas Potgieter - Humanized Pichia pastoris
4. Don Bergmann, PhD - Building Human Replacement Organs
5. Barbara Immel - cGMPs for QC Laboratories


• BIOMAN 2008 (none)


• BIOMAN 2009
1. Brian Roach - Corn Based Ethanol in Fulton, NY
2. Carol Tomet - Future of Micronucleus Training
3. William Trompeter – Pharmaceuticals: History, Science and Technology
• BIOMAN 2011
1. Lee Landeen, Ph.D. - Engineered Tissues for Wound Healing Applications
2. Stephen Mayfield, Ph.D - Biofuels

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OTHER VIDEO RESOURCES:

ATETV Biomanufacturing Videos
• What is Biomanufacturing
• Student Profile
• Products From Living Cells
• What Is An SOP?
• Community College Programs
• Influencing Quality of Life

Focus On Biotechnology
A DVD Video produced by Minuteman Regional High School
A very polished fast pace 15 minute video illustrating the demand and nature of technician training to meet the growing shortage of biotechnicians in the US. A useful tool to promote interest in biotechnology as a career field among high school science students and undeclared college students. Click here to go to the online request form.

BIOMAN 2007 Participant Interviews
Click here and view this thirteen minute video of participant interviews speaking about why they came to the BIOMAN conference and how they benefitted from the experience.

Quality Control Microbiology: What Do Technicians Do?
Guest lecturers from Lonza Biologics delineate various roles of QC Microbiology technicians and their relationship in the biomanufacturing process.
Time = 72:41 File size = 33 meg
View or download the web video here.

Quality Control Biochemistry: What Do Technicians Do?
Guest lecturers from Lonza Biologics delineate various roles of QC Biochemistry technicians and their relationship in the biomanufacturing process.
Time = 68:41 File size = 61 meg
View or download the web video here.

Media Preparation for E. coli Culture
A training video developed by Deb Audino and Robert O'brien at NHCTC Biotechnology department.
Time = 5:41 File size = 9.1 meg
View or download the web video here.

NBC² Merchandise
     View and order Biotechnology Acronyms housewares on CafePress.com

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